Morphological and Functional Characterization of Hemocytes of the Black Sea Bivalves: Pacific Oyster (Crassostrea gigas) and Mussel (Mytilus galloprovincialis)

To understand the role that hemocytes play in processes of cellular immunity bivalve mollusks, they should be accurately classified based on their morphological and physiological characteristics. The circulating cultured Pacific oyster (Crassostrea gigas) marine mussel (Mytilus galloprovincialis) were investigated using light microscopy flow cytometry. In mussel, two cell types, granulocytes agranulocytes, identified presence subpopulations cells differing by size granularity level scatter plots. Light microscopic examination confirmed with cytoplasmic granules without granulation hemolymph mussel. oyster, cytometry revealed three types hemocytes: hyalinocytes, granulocytes. both species mainly represented agranular cells, which constituted 78.4 ± 8.9 % 86.7 2.7 (agranulocytes hyalinocytes) oyster. Agranulocytes smallest type They round-shaped had large nuclei narrow cytoplasm. Hyalinocytes larger irregularly shaped, eccentric nuclei. Granulocytes contained numerous eosinophilic, basophilic, mixed formed pseudopodia. Flow showed produced considerably fewer reactive oxygen compared to Morphological functional characterization cultivated improves analysis state mollusks farmed Black Sea region